Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Both readers were heated to 37ºC prior to setting up the assay. for ValitaTiter Plus standards read on Molecular Devices readers. (12) Variants in ASPH cause exertional heat illness and are associated with malignant hyperthermia susceptibility Nature CommunicationsDecember 1, 2022 Yukari Endo, Linda Groom, Alper Celik, Natalia. Step 1: Turn on your microplate reader. style. Resipher 96x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 96x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 96 wells Utilizes Lucid Lab Cloud software for connection to any…. Reactions catalyzed by firefly and *Renilla luciferases. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. Dynamic range > 9 orders of magnitude. best-in-class data . A 1:10 serial dilution of ATP was prepared in medium. 4 Pages. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ EMax Plus R2 = 0. Fluorescent DNA quantitation. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. SpectraMax iD5. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and time-resolved fluorescence (50-1450 µsec, 200 µsec increments) measurements in 6, 12, 24, 48, 96, and 384 well microplates or in. We demonstrate that miniaturizing the assay in 96-well half area microplates, and using the flexible liquid transfer features of the FlexStation ® 3 Multi-Mode Microplate Reader allows rapid and reliable measurement of EC 50 values for agonists and IC 50 values for antagonists. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. July 17, 2023. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Highly selective for dsDNA over ssDNA or RNA. In labs where throughput is a top priority, a device that can screen 96- to 1536-well microplates in under one minute is worthwhile. 2 mm x 86 mm) Height:. #T-0254) • Lysozyme (Sigma cat. 4. Retrieving. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Type: Microplate Reader. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. 3:40 . • SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. M5. 96, 384, 24, 12, 6 well types. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read: black adapter (p/n: 0310-4089) Flex mode always uses black adapter because it's always bottom read. Optical System: High energy xenon flash lamp. 50 racks/case for 384-well (16 channel pipettor head. A single-mode plate reader is a good starting point. 6. Molecular devices The VersaMax Absorbance Microplate Reader costs between $5,500 and $6,600. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. Endpoint, kinetic, spectrum and area-well scanning Click here. The reader adds up to three transfers of assay reagents from a 96- or 384. Simple, economical . #I2511)Introduction. It features a modular design that allows users to add or change detection cartridges according to their needs. This page contains the. 1700 Online: Sales Offices. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. This document outlines references to 21 CFR Parts 58, 211 and 820 and EudraLex Annex 15 to assess the implementation of Molecular Devices microplate readers in regulated environments. 5. AquaMax 4000 Microplate Washer (Molecular Devices Cat. ValitaTiter Plus 96-well (1 plate) VAL004. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. i3x Multi-Mode Microplate Reader. Infinite 200 Pro Microplate Reader. Four wash/rinse bottles are included and configurable for both 96- and 384. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. We would like to show you a description here but the site won’t allow us. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Supplementary Information. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. 4 nm Microplate Capacity Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates Incubation Temperature control range from Ambient +4º to 50ºC Temperature variation ± 0. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. ValitaTiter 96-well (Pack of 20). 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. SpectraMax® Microplate Reader Suite of Products Knowledge Base. The AquaMax® Microplate Washer is a fully self-contained system, configurable for both 96- and 384-well microplates. Keywords: fluorescence polarization, trf, fret, igg. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Geoffrey Vrla, a molecular biology graduate student at Princeton, found Tecan's Infinite 200 Pro Microplate Reader to be a versatile and accurate instrument for quantifying fluorescent proteins expressed in bacteria. 1-color 96-well . In addition, SoftMax Pro SoftwareUsing Molecular Devices microplate readers and SoftMax. Microplates . 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. *Simple analysis and optimization. #G7294). changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Equipped with scanning, shaking and kinetics modules. 采用ELISA 方法，对比Molecular Devices 与某进口品牌中等吸附和高等吸附96 孔全透明微孔板，结果表明两种品牌高等吸附板都能结合更多的Protein A，同时Molecular Devices 全透明微孔板有更优的复孔重复性。 The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. This . Related Products: Low Binding Plates. Temperature Control: ambient from +4 to 45C. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. SoftMax Pro Software’s. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Plate reader instructions Computer. in space. Plate type 96-well standard clear bottom: Timing Total run time: 01:40:00: Interval: 00:01:00 More settings : Carriage speed: Slow:The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Materials. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Standard features on the EMax® Plus Microplate Reader include the ability to measure both flat and round 96-well microplates, automatic lamp calibration prior to each reading (ensuring automatic measurements), powerful curve fitting protocols and statistical analysis tools, and integrated instrument control and data analysis with SoftMax® Pro. Setup Guide. Description. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. flat-bottom: 50-60 µl. Molecular Devices microplate readers have the most variety of tools available for users to meet their diverse hardware and software validation needs. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Protein quantitation Cross-talk < 0. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. Detection modalities are shown in Table 1-1. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. Both COC microplates, the μCLEAR UV STAR 96- and 384-well plates, had background absorbance of 1. 003 fmol 0. of 0. The VMax Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm-750nm), with 4 filter choices included at time of purchase. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. For more sample throughput, the system canRating: 3. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Or scan up to 384 wells at any time to determine the best assay conditions. Microplates . For more sample throughput, both readers can be easily integrated into full roboticPrimary considerations when choosing a microplate reader are throughput and flexibility. to each well. 100-μL samples ranging from 1 nM to 10 μM were added to triplicate wells of a solid white 96-well microplate. Five-second read per plate. 7. reagent basin for ease of pipetting into. Easy-to-use, intuitive, configurable microplate readers with industry-leading, SoftMax Pro microplate software solution for increased productivity. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurements in the. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. 8 Pages. 5°C at 37°C. While "plate reader" usually refers to the devices described. Softmax Pro GxP Software. This can increase throughput and efficiency, thus saving time and space in the lab. Category 2 and 3 microplate readers use gradient heating as well. Abstract and Figures. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Fax 4200 BERTHOLD TECHNOLOGIES Lumat LB 9507 Tecan SpectraFluor Thermo Scientific Varioskan LUX LJL Biosystems Analyst AD 96. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. 1 mL/well of media. The validation kit included individual tubes of diluted fluorophores:. 100 fmol 1536-well (8 µL) 0. MycoAlert Reagent was added to each well and incubated at room temperature for 5. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. . This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. Three low volume 24-well bottom slides. Description. Standard SBS 96 and 384 well plates (128. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. #SpectraMax L Config) Methods. 01. Endpoint, kinetic, spectrum. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. 384 well polystyrene microplates have plate dimensions of 127. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96- or 384-well head. Instrument settings are listed in Table 1. 5% at 1. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. Molecular Devices announced today the launch of the new SpectraMax ® ABS and ABS Plus Microplate Readers that cover a wide range of absorbance-based applications such as ELISAs, microbial growth, and protein quantitation and can be easily integrated into full robotic systems. Paradigm: Delta mP. EarlyTox Live/Dead Assay Kit (Molecular Devices cat. It features a modular design that allows users to add or change detection cartridges according to their needs. This unlocks the transport slide. Accuris MR9600 SmartReader 96 Microplate Absorbance Reader. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs) Specifications/Features: Shaking, Temperature control, Injector. Supplier: Molecular Devices. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. Photometric and kinetic analysis mode. Please refer to the Obsolete Microplate Reader Models for more information. Plate readers, also known as microplate readers or microplate photometers,. 4D-Nucleofector 96-well Shuttle: RATE CONTROLLED FREEZING: Grant: Asymptote EF600M Freezer:. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. ScanLater Western Blot Detection System. In . Brand: MOLECULAR DEVICES. F3/F5 Multi-Mode Microplate Readers. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. explore more. #iD5-STD) Methods ATP standard curve. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. #PLUS 384) Methods Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. Microplate readers from Molecular Devices are designed to provide consistent performance over a long period of time. SoftMax Pro Software’s SpectralThe Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Contents of wells were pipetted up and down several times to mix. Whatever your needs, you’ll find a microplate reader that can meet them. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. We would like to show you a description here but the site won’t allow us. After 5, 7, 11, and 13 days. and luminescence microplate reader validation DATA SHEET. Can be integrated with Molecular Devices' StakMax® Microplate Stacker. ©2010 molecular Devices, inc. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. Technical specifications. A preconfigured. Microplate Readers. validation, maintenance, and repair documentation. BSA concentration (µg/mL) Average OD. Molecular Devices 1311 Orleans Drive Sunnyvale, California 94089 Telephone: +1-408. U-bottom: 30-40 µl. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. 1 OD at 230 nm. 2. Cells were washed three times with calcium wash buffer using an AquaMax 4000 Microplate Washer with 96- or 384-well cell wash head. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. ) 20,000 10,000 30,000 40,000 50,000 60,000 70,000 80,000 0. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Taking into account the price differences between each seller and each country. 2 Pages. Flexible Microplate Format The GEMINI system allows you to select from 6-, 12-, 24-, 48-, 96- and 384-well microplate formats for your fluorometric measurements. The IC design and microplate construct represents a major advancement to state-of-the-art CMOS devices for biological measurement, where the large majority are. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid-Molecular Devices Tunable Microplate Reader VersaMax $ 1,495. Prior to the reading of each plate, the microplate reader was “blanked” with a plate containing 0. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. What plate adapters do you recommend for 96, 384-well plates for FlexStation? Flex/Flex II/384: Top read: purple adapter (p/n: 0310-4336) Bottom read:. The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise results. 3 OD at 200 nm. Molecular Devices' microplate readers. In this article, Biocompare product reviewers share why the Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader is one of the best on the market. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. These robust readers have been placed in labs from. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. Minneapolis, MN, USA), according to the manufacturer's protocol. 0 OD at. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. No additional manuals are available. SOFTmax PRO allows you to select excitation and emission values from the literature and simply input their values for the monochromator settings. Optimized optical path - The reader’s unique quartz fiber optic system minimizes stray light for precise readings across a microplate. Detergent Compatible Bradford assay. StakMax. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. Measuring Time: Reading Times (Flying Mode 1 flash): 9 sec (96), 16 sec (384) Wavelength Range:Both the MTT and Cell Cytotoxicity assays were read on the SpectraMax® ABS Plus Microplate Reader, which can read a 96-well microplate in less than 10 seconds. Infinite 200 Pro Microplate Reader. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. You can add your own protocols. The SpectraMax® ABS Plus Microplate Reader can run . Molecular Devices M5 Plate Reader Plate Calibration Form . Figure 2. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Five preferred modes for diverse bioresearch applications. 54: 1. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Microscopy. the assay. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Primary Disinfectant (Cavicide, Warehouse, Cat # 79300360, or equivalent) 6. About;. No accessories are needed and SoftMax® Pro Software. the appropriate well of the microplate. #3676). 10. ***Figure 1. Seller assumes all responsibility for this listing. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Operates as a stand-alone reader or with input/output stackers and bottom reading optics. Integrated stacker option. 3. Page 154: Ordering Parts And Accessories. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W (ABS Plus) • Option to run samples as low as 2 μL with the SpectraDrop™ Micro-Volume Microplate • Runs various assays within the Vis/UV-Vis range such asI tried Nunc Edge 2. Compact design Minimum volume and. Built in near field communication (NFC). The ThermoMax performs automatic mixing before a reading and between readings to ensure reliable and precise. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. VersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. Detection by Low noise PMT with simultaneous digital and analog modes. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. New SpectraMax iD5 Multi-mode Microplate Reader. The UV-Vis SpectraMax 190 Microplate Reader for Quantification of DNA and endpoint, kinetic, and spectral scanning analyses. 19 H mm that meet microplate industry standards. Step 2: Launch SoftMax Pro Software. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. Alpha Technology capability can be added to the system at any time as a user’s detection needs evolve. Here, we show that Molecular Devices microplate readers can easily and noninvasively measure fluorescent proteins in living cells. The StakMax® Microplate Handling System is a powerful, compact, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers for improved process flow and higher throughput. This paper describes a robust and rapid procedure for measuring chlorophyll using a microplate reader. 3. The SpectraMax Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. 0 500 1000 1500 2000 2500 3000 3500 time (sec. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature. 500 USD. Molecular Devices 推出了FlexStation® 3 多功能读板机系统。FlexStation 3 把 SpectraMax M5e 和 8 道 / 16 道的移液 系统整合在一起成为体积小巧功能强大的 读板机系统。该整合系统不但为用户提供 了多功能检测平台，还增加了液体处理的 通量和提升了检测生化和细胞动. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. We obtained three HEK-293 cell lines from Clontech, each stably transfected with a different fluorescent protein. A. Published Dec 16, 2020 | Updated Dec 16, 2020. Please click on the links below to view the Microplate Reader Comparison Tables. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. 2 2600A ValitaTiter. Well scanning With well scanning, multiple points within each well can be read, providing a high. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. ZERO BIAS. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. For more sample throughput, the system canMolecular Devices LLC 96 well microplate reader spectrophotometer 96 Well Microplate Reader Spectrophotometer, supplied by Molecular Devices LLC, used in various techniques. 96-well flat clear bottom white TC-treated microplates (Corning cat. SpectraMax Plus 384 and 340PC 384 readers can accommodate standard 96-well, 384-well, and strip-well microplates. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. • 0. Reading a Microplate . 375 pM. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. 2 OD at 200 nm; this decreased to 0. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. softmaxt pro microplate data analysis, molecular devices’ stakmax microplate stacker, monochromator-based fluorimeter, wavelength optimization, fda 21 cfr part 11. 0 500 1000 1500 2000 2500 3000 3500 time (sec. The SpectraMax L reader is easily integrated with the optional StakMax microplate stacker from Molecular Devices for automated multiplate assays. In simplest terms, a plate reader measures and. The SpectraMax® i3x Multi-Mode Microplate Reader measures spectral-based . we demonstrate how Molecular Devices microplate readers with luminescence detection mode provide superior sensitivity and ease of use for reliable mycoplasma detection using MycoAlert. Average StDev (mP) 1. The microplate washer automatically washes all wells of the assay plate, using the desired wash program to completely remove unbound reagents and eliminate background noise. All readings were taken in clear, polystyrene 96-shallow-well, flat-bottom plates. Molecular Devices is a leader in comprehensive compliance solutions with microplate detection systems. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. #W50-100) SpectraMax® ABS Plus Microplate Reader (Molecular Devices cat. Filters: Excitation and emission slides for 4 filters each. 012 fmol 0. (Molecular Devices cat. measurements in compatible Molecular Devices® microplate readers. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. The drawing shows a standard microplate of 15 mm height covered with a lid. Add 150 µL reagent to each well. The procedure enables direct translation of any published equation. The official tips for the FlexStation® 3 Series Multi-Mode Microplate Readers system. 96-well microplate at 200 µL/well. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. SpectraMax iD3 Multi-Mode Microplate Reader (Molecular Devices cat. #I2511) • Assay diluent – may be cell culture medium. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Flexible for 6-well to 384-well microplates and cuvettes. Both readers were heated to 37ºC prior to setting up . Published Dec 16, 2020 | Updated Dec 06, 2021.